#Xtractor clontech buffer plus

Thermo Scientific Y-PER Plus Dialyzable Yeast Protein Extraction Reagent is a phosphate-free, low-salt formulation with a dialyzable detergent, validated for use primarily with S.

Although the detergent is compatible with various downstream methods, it is not dialyzable, so it cannot be removed in cases where incompatibility exists. pastoris (as well as various gram-positive and gram-negative bacteria). Y-PER reagent is high salt (>300 mM) and is effective for S. Two formulations are available depending on the downstream application. Y-PER reagent provides higher yields and greater flexibility for use in current proteomics workflows. Traditionally, yeast protein extraction required mechanical disruption with glass beads, making small-scale extraction difficult. Thermo Scientific Y-PER Yeast Protein Extraction Reagent penetrates the tough yeast cell wall, perforating the cell wall and membrane and extracting soluble protein without completely damaging overall cell structure. Western blotting, 6xHis-tagged protein purification, protein assays, ion-exchange chromatography SDS or native-PAGE, western blotting, immunoprecipitation, affinity purification, activity assays SDS-PAGE, western blotting, protein purification, activity assays Gentle, effective protein extraction without sonication Obtain excellent protein yields in less than 10 minutes using a single buffer and a spin column Suspended or adherent cultured insect cells (Sf9, Sf21)Дoes not require glass beads for extraction Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, Bacillus subtilis, Escherichia coli Saccharomyces cerevisiae, Schizosaccharomyces pombe and Pichia pastoris I-PER Insect Cell Protein Extraction Reagent Pierce Plant Total Protein Extraction Kit Y-PER Plus Dialyzable Yeast Protein Extraction Reagent These reagents provide high protein yield and typically allow samples to be processed in 10вЂ“15 minutes. Protein extracts are compatible with most protein assays and typical downstream applications. Thermo Scientific B-PER Complete Bacterial Protein Extraction Reagent is an all-in-one formulation that combines a lysis reagent with lysozyme and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria.

Several different ready-to-use formulations of B-PER reagent are available for different lysis needs. B-PER reagent removes soluble protein from inclusion bodies and can be used to purify intact inclusion bodies whose less soluble proteins can be extracted by other means. Extraction does not require expensive equipment or mechanical disruption and can be performed in less than 10 minutes. The reagent is also suitable for certain Archaebacteria species and cultured insect cells. B-PER reagents can be used for Gram-negative bacteria, S. coli and other species of bacterial cells and effectively extract soluble native and recombinant proteins. Thermo Scientific B-PER Bacterial Protein Extraction Reagents gently lyse E.

SDS-PAGE, western blotting, protein purification (except for GST fusion proteins) SDS-PAGE, western blotting, protein purification No, but for gram-positive bacteria add enzymes for increased yield Yes, add EDTA to gram-negative fresh cells View recommended protein preparation buffers for mass spectrometry applicationsД«-PER Complete Bacterial Protein Extraction ReagentД«-PER Bacterial Protein Extraction ReagentД«-PER II (2X) Bacterial Protein Extraction ReagentД«-PER (PBS) Bacterial Protein Extraction ReagentД«-PER reagent formulated in Tris buffer with enzymesДўX B-PER reagent formulated in in Tris buffer IP, protein electrophoresis, western blots, ELISA, EMSA, reporter assays, purification, activity assays, amine reactive labeling Non-denaturing detergent in 25 mM bicine buffer (pH 7.6)ДЄ modified RIPA buffer formulation without SDSД«CA assays, Detergent Compatible BradfordД®LISA, protein electrophoresis, western blotting Mild, non-denaturing and efficient lysis for cytoplasmic and nuclear protein extractionД®xtraction of cytoplasmic and nuclear protein in denaturing conditionsДЇormulated specially for pull-down and immunoprecipitation assays Need a harsher buffer than NP-40 or when cytoplasmic and nuclear protein extraction is needed Recommended for extraction of cytoplasmic proteins M-PER Mammalian Protein Extraction Reagent

Xtractor clontech buffer